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KMID : 0363320110320040530
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Journal of Korean Oriental Internal Medicine
2011 Volume.32 No. 4 p.530 ~ p.541
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The Effect of Ethanol Extracts of Herba Prunellae, Flos Lonicerae and Radix Scutellaria on 6-OHDA Induced Oxidative Damage in the SH-SY5Y Cell Line
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Min Kwan-Sik
Kim Su-Young
Kim Min-Woo
Lee Key-Sang
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Abstract
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Objectives: Categorized as ’cheongyeol’ herbs, Herba Prunellae, Flos Lonicerae and Radix Scutellaria have been proven to have effect on degenerative brain disease, cerebrovascular disease and brain tumor because of their anti inflammation, antioxidant, or anticancer effects. In this study, we studied activity against reactive oxygen species and anti inflammation effect of these three ’Cheongyeol’ herbs.
Methods: We measured each herb’s yield of ethanol extracts, phenolic contents and activities against DPPH, hydroxyl radical and superoxide anion. Also through 6-hydroxydopamine (6-OHDA) induced oxidative damage in SH-SY5Y human neuroblastoma cell line, we measured antioxidant effect and NO activity of the three herbs. From the three herbs, we chose Prunella Herba, which showed the highest antioxidant effect, and studied its cell survival rate and anti inflammation effect through COX-2 and iNOS.
Results: All three herbs showed significant results, and especially Prunella Herba showed significant effect on phenol contents, antioxidant effect on various active oxygen and antioxidant, and anti inflammation effect through cell line.
Conclusions: Further study of the origin concept of ’cheongyeol’ and research into specific mechanisms and role in treatment of cranial nerve disease, seems warranted.
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KEYWORD
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reactive oxygen species, Herba Prunellae, Flos Lonicerae, Radix Scutellaria, antioxidant effect
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